Journal: Scientific Reports

Article Title: Glucose coated FeO@Fe 3 O 4 nanoparticles show tunable catalytic reactivity and safety in a 3D hepatic in vitro model

doi: 10.1038/s41598-026-52667-5

Figure Lengend Snippet: Potential induction of DNA double-strand breaks (DSBs) and p-H3 positive events by glucose-coated FeO@Fe 3 ​O 4 MNPs in HepG2 spheroids. ( A ) The fluorescence intensity of γH2AX was measured in individual cells by flow cytometry and normalized to the vehicle control (VC; up to 3% Milli-Q water in cell culture media), represented by dotted line. Cells were exposed to increasing concentrations of the nanoparticles for 24 h (up to 20 µg/cm²) and 96 h (up to 2 µg/cm²). Etoposide (1.7 µM) served as a positive control (PC). Statistically significant differences between MNPs–treated cells and the VC were determined using ANOVA, followed by Dunnett’s post-test and are indicated as **** P < 0.0001. ( B ) The average percentage of p-H3–positive events, indicative of mitotic activity, was also determined by flow cytometry following exposure to graded concentrations of the nanoparticles for 24 h (up to 20 µg/cm²) and 96 h (up to 2 µg/cm²). Colchicine (0.1 µM) was used as a positive control. Significant differences compared to the VC (up to 3% Milli-Q water in cell media) were identified by ANOVA and Dunnett’s post-test and are marked as **** P < 0.0001.

Article Snippet: The following antibodies were acquired from Miltenyi Biotech (Germany): γH2AX pSer139-APC (130-123-256) and pH3-PE (130-105-700).

Techniques: Fluorescence, Flow Cytometry, Control, Cell Culture, Positive Control, Activity Assay

Journal: Cell Death Discovery

Article Title: Novel imidazotetrazine derivatives overcome temozolomide resistance in glioblastoma by inducing ferroptosis and apoptosis

doi: 10.1038/s41420-025-02857-3

Figure Lengend Snippet: Immunofluorescence staining for γH2AX was performed on U251 cells ( A ) treated with 10, 20, and 40 μM QX302, U87 cells ( B ) treated with 20, 40, and 80 μM QX302, and T98G cells ( C ) treated with 50, 100, and 200 μM QX302 for 72 h.DMSO was used as the negative control and TMZ as the positive control. The accompanying graphs show the statistical analysis of the proportion of γH2AX-positive nuclei. D Western blot analysis of ATM, p-ATM, γH2AX, and GAPDH (internal reference) protein levels in U251, U87, and T98G cells treated with QX302 for 72 h. E Linearized pUC19 plasmid DNA (100 ng) was treated with various concentrations of QX302 at 37 °C for 15 h, followed by denaturing gel electrophoresis with cisplatin as the control. F The effect of QX302 and TMZ (positive control) on plasmid DNA under different denaturing conditions. G The effect of QX302 on DNA alkylation when assayed under different experimental conditions. Tris = Tris buffer (pH 8.5); H 2 O = unbuffered water (pH 7); 0.5/1 = 0.5% or 1% NaOH were used to denature pUC19 plasmid DNA. The data shown represent the means ± SD from three independent experiments. ns > 0.05, * p < 0.05, ** p < 0.01 and *** p < 0.001, compared to control group.

Article Snippet: Antibodies against GAPDH (5174S), caspase 3 (9662S), cleaved caspase 3 (9661S), caspase 7 (9492S), cleaved caspase 7 (9491S), caspase 9 (9502S), cleaved caspase 9 (9505S), cleaved PARP (5625S), CDK1 (CDC2, 9116S), p-CDK1 (p-CDC2, 9114S), cyclin B1 (12231 T), P53 (2524S), ATM (2873 T), p-ATM (4526 T), and γ-H2AX (80312S), and anti-rabbit IgG, HRP-linked antibody (7074S), and anti-mouse IgG, and horseradish peroxidase (HRP)-linked antibody (7076S) were procured from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Immunofluorescence, Staining, Negative Control, Positive Control, Western Blot, Plasmid Preparation, Nucleic Acid Electrophoresis, Control